Activities of bacteriocin from Pseudomonas putida strain FStm2 against biofilm- forming bacteria isolated from urinary catheter

Aims: The aim of the present study is to investigate the effect of bacteriocin from Pseudomonas putida FStm2 on biofilm-forming bacteria isolated from urinary catheter. Methodology and results: In this study, 25 bacteria isolates were successfully isolated from urinary catheter. Sixteen of the isolates were urease positive. Results of crystal violet test showed that 8 isolates were active biofilm formers in microtiter plate. Seven isolates were active biofilm formers in urinary catheter filled with nutrient broth (NB) and 9 isolates formed biofilm in catheters filled with artificial urine. Bacteriocin-producing Pseudomonas putida strain FStm2 was isolated from shark skin. The antibacterial spectrum of the bacteriocin was determined using the well diffusion method and activity was evident against three Burkholderia cepacia isolates and one Staphylococcus hominis isolate from urinary catheter. Conclusion, significance and impact study: Bacteriocin from P. putida FStm2 has good antimicrobial activity against B. cepacia and S. hominis. It can be a good candidate as anti-biofilm in combating urinary catheter infection. Further studies such as the mechanism of action of the bacteriocin against both bacterial species should be studied in detail if it is to be developed as a bacteriocide.